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alexa fluor 647 conjugated affinipure donkey anti igg  (Jackson Immuno)


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    Structured Review

    Jackson Immuno alexa fluor 647 conjugated affinipure donkey anti igg
    Alexa Fluor 647 Conjugated Affinipure Donkey Anti Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/donkey+anti+human+alexa+fluor+647/Alexa+Fluor+647+AffiniPure+Donkey+Anti-Human+IgG/pmc12995288-163-15-21
    Average 94 stars, based on 117 article reviews
    alexa fluor 647 conjugated affinipure donkey anti igg - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    other:

    Article Title: CRL4 DCAF12 regulation of MCMBP ensures optimal licensing of DNA replication
    Article Snippet: Secondary antibody conjugates for IF were goat anti-rabbit Alexa Fluor 488 (A11034), goat anti-rabbit and goat anti-mouse Alexa Fluor 568 (A11036, A11031), goat anti-rabbit and goat anti-mouse Alexa Fluor 647 (A21245, A21236) (all from Thermo Fischer Scientific, 1:2,000); donkey anti-human Alexa Fluor 647 (Jackson Immuno Research, 709-605-149, 1:2,000); goat anti-mouse (DyLight 488, Thermo Fisher, 35503, 1:1,000); and donkey anti-rabbit IgG (Alexa Fluor® 555, Abcam, ab150070, 1:1,000).

    Article Title: Elimination of separase inhibition reveals absence of cohesin protection in oocyte metaphase II
    Article Snippet: The following secondary antibodies were used at 1:200: donkey anti-human Cy3 secondary antibody (Jackson Immuno Research, 709-166-149), donkey anti-human Alexa Fluor 647 (Jackson Immuno Research, 709-606-149), donkey anti-rabbit Cy3 (Jackson Immuno Research, 711-166-152), donkey anti-rabbit Alexa Fluor (Jackson Immuno Research, 711-546-152), donkey anti-mouse Alexa Fluor 488 (Jackson Immuno Research, 715-546-150).

    Article Title: Development of a cell-permeable Biotin-HaloTag ligand to explore functional differences between protein variants across cellular generations
    Article Snippet: For IF, secondary antibody conjugates were goat anti-rabbit Alexa Fluor 647 (Thermo Fischer Scientific, A-21245, 1:2000) and donkey anti-human Alexa Fluor 647 (Jackson Immuno Research, 709-605-149, 1:2000).

    Article Title: Eliminating separase inhibition reveals absence of robust cohesin protection in oocyte metaphase II
    Article Snippet: The following secondary antibodies were used at 1:200: donkey anti-human Cy3 secondary antibody (Jackson Immuno Research, 709-166-149), donkey anti-human Alexa Fluor 647 (Jackson Immuno Research, 709-606-149), donkey anti-rabbit Cy3 (Jackson Immuno Research, 711-166-152), donkey anti-rabbit Alexa Fluor (Jackson Immuno Research, 711-546-152), donkey anti-mouse Alexa Fluor 488 (Jackson Immuno Research, 715-546-150).

    Article Title: Eliminating separase inhibition reveals absence of robust cohesin protection in oocyte metaphase II
    Article Snippet: Donkey anti-human Alexa Fluor 647 , Jackson Immuno Research , 709-606-149.

    Article Title: CRL4 DCAF12 regulation of MCMBP ensures optimal licensing of DNA replication
    Article Snippet: Secondary antibody conjugates for IF were goat anti-rabbit Alexa Fluor 488 (A11034), goat anti-rabbit and goat anti-mouse Alexa Fluor 568 (A11036, A11031), goat anti-rabbit and goat anti-mouse Alexa Fluor 647 (A21245, A21236) (all from Thermo Fischer Scientific, 1:2,000); donkey anti-human Alexa Fluor 647 (Jackson Immuno Research, 709-605-149, 1:2,000); goat anti-mouse (DyLight 488, Thermo Fisher, 35503, 1:1,000); and donkey anti-rabbit IgG (Alexa Fluor® 555, Abcam, ab150070, 1:1,000).

    Incubation:

    Article Title: Dual targeting of transferrin receptor and CD98hc enhances brain exposure of large molecules.
    Article Snippet: After blocking, sections were incubated overnight at 4◦C with the following primary antibodies, diluted in 5% BSA +0.3% Triton X-100 (dilution buffer): rabbit anti-TfR (Abcam, ab214039) and anti-NeuN (GeneTex, GTX00837, 1:500) or human anti-CD98hc (Denali, 1:1000) and anti-AQP4 (Millipore, AB2218, 1:500). .. Following primary antibody incubation, sections were washed three times with PBS and then incubated overnight at 4◦C with the following secondary antibodies + DAPI (ThermoFisher Scientific, D1306) in dilution buffer: donkey anti-rabbit-Alexa Fluor 647 (1:500 ThermoFisher Scientific, A31573) and donkey anti-chicken-Alexa Fluor 488 (1:500, Jackson ImmunoResearch, 703- 545-155) or donkey anti-human-Alexa Fluor 647 (1:500 Jackson ImmunoResearch, 709-606-149) and donkey anti-rabbit-Alexa Fluor 488 (1:500, ThermoFisher Scientifi A21206). .. Sections were then washed three times with PBS and mounted in ProLong Glass antifade mounting media (ThermoFisher Scientific, P36984).

    Immunofluorescence:

    Article Title: Quantitative AI-based DNA fiber workflow to study replication stress
    Article Snippet: For immunofluorescence, the following primary antibodies were used: GFP (rabbit, Chromotek, PABG1, 1:1000), TIMELESS (rabbit, Abcam, ab109512, 1:1000), PCNA (human, Immuno Concepts, 2037, 1:1000), RAD51 (rabbit, BioAcademia, 70-012, 1:1000). .. Secondary antibodies for immunofluorescence: goat anti-rabbit Alexa Fluor 568 (Thermo Fisher Scientific, A-11036, 1:2000), goat anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific, A-11034, 1:2000), and donkey anti-human Alexa Fluor 647 (Jackson Immuno Research, 709-605-149, 1:2000). .. For Western Blot, the primary antibodies used were BRCA2 (mouse, Merck, OP95, 1:1000), MCM7 (mouse, Santa Cruz, sc-9966, 1:1000), GFP (rabbit, Chromotek, PABG1, 1:1000), and vinculin (mouse, Merck, V9131, 1:20000).



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